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mesc medium knockout dmem thermo fisher scientific 10829018  (Thermo Fisher)


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    Thermo Fisher mesc medium knockout dmem thermo fisher scientific 10829018
    a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 <t>knockout</t> (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ <t>mESC</t> colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).
    Mesc Medium Knockout Dmem Thermo Fisher Scientific 10829018, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesc medium knockout dmem thermo fisher scientific 10829018/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    mesc medium knockout dmem thermo fisher scientific 10829018 - by Bioz Stars, 2026-03
    86/100 stars

    Images

    1) Product Images from "Extraembryonic gut endoderm cells undergo programmed cell death during development"

    Article Title: Extraembryonic gut endoderm cells undergo programmed cell death during development

    Journal: Nature Cell Biology

    doi: 10.1038/s41556-024-01431-w

    a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 knockout (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ mESC colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).
    Figure Legend Snippet: a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 knockout (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ mESC colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).

    Techniques Used: Knock-Out, Fluorescence, Microscopy, Generated, Comparison, Standard Deviation, Flow Cytometry, Isolation



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    mesc medium knockout dmem thermo fisher scientific 10829018  (Thermo Fisher)
    86
    Thermo Fisher mesc medium knockout dmem thermo fisher scientific 10829018
    a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 <t>knockout</t> (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ <t>mESC</t> colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).
    Mesc Medium Knockout Dmem Thermo Fisher Scientific 10829018, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesc medium knockout dmem thermo fisher scientific 10829018/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    mesc medium knockout dmem thermo fisher scientific 10829018 - by Bioz Stars, 2026-03
    86/100 stars
    		  Buy from Supplier
    mesc medium [knockout-dmem  (Thermo Fisher)
    90
    Thermo Fisher mesc medium [knockout-dmem
    a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 <t>knockout</t> (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ <t>mESC</t> colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).
    Mesc Medium [Knockout Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesc medium [knockout-dmem/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mesc medium [knockout-dmem - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 knockout (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ mESC colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).

    Journal: Nature Cell Biology

    Article Title: Extraembryonic gut endoderm cells undergo programmed cell death during development

    doi: 10.1038/s41556-024-01431-w

    Figure Lengend Snippet: a ) Schematic illustrating the two-colour lineage tracing strategy combined with extraembryonic lineage-specific p53 knockout (KO). The mCherry+ zygote is electroporated with Cas9/gRNA complex, and once reaching the pre-compaction morula stage, the p53 KO embryo is aggregated with a GFP+ mESC colony. As a result, the extraembryonic lineages are p53 KO, including the mCherry+ gut cells of extraembryonic origin, while the GFP+ embryonic lineage is wild type. b ) Bright-field and fluorescence microscopy images of an E9.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO ( n = 5, one representative embryo is shown). c ) Bright-field and fluorescence microscopy images of an E13.5 embryo and its corresponding yolk sac, generated via the two-colour lineage tracing combined with extraembryonic lineage-specific p53 KO. The dissected intestine was manually separated into the colon and small intestine, indicated by the black line. The overall GFP+ intestine contains mCherry+ cells, indicated by the white arrows ( n = 3, one representative embryo is shown). d ) EPCAM+ content of WT lineage-traced embryos, showing the posterior part of E9.5 embryos, and the colon and small intestine from E13.5 embryos (*WT data from Extended Data Fig. used here as comparison, n = 9). Additionally, EPCAM+ content of lineage-traced embryos with extraembryonic-specific p53 KO is presented showing the posterior part of E9.5 embryos ( n = 5) and the colon and small intestine from E13.5 embryos ( n = 3). Bars denote the mean, error bars denote the standard deviation, and single replicates are indicated by dots. e ) Flow cytometry dot plots of the corresponding colon (left) and small intestine (right) from an E13.5 lineage-traced embryo with extraembryonic lineage-specific p53 KO showing the endoderm fraction (EPCAM+, top) and non-endoderm fraction (EPCAM−, bottom) of the isolated organs ( n = 3). mCherry+ cells with extraembryonic origin are detected. f ) Flow cytometry dot plot of the limb from an E13.5 lineage-traced embryo ( n = 3).

    Article Snippet: The mESCs were cultured on gelatinized plates coated with mitotically inactive primary CD-1 mouse embryo fibroblasts (MEFs) in the presence of serum and LIF in mESC medium (KnockOut DMEM (Thermo Fisher Scientific, 10829018), 15% fetal bovine serum (FBS; Thermo Fisher Scientific, 16140071), 1×GlutaMAX supplement (Thermo Fisher Scientific, 35050038), 1×non-essential amino acids (Thermo Fisher Scientific, 11140035), 1:1,000 2-mercaptoethanol (Thermo Fisher Scientific, 21985023), 1×penicillin–streptomycin (Thermo Fisher Scientific, 15140122) and laboratory-purified recombinant LIF).

    Techniques: Knock-Out, Fluorescence, Microscopy, Generated, Comparison, Standard Deviation, Flow Cytometry, Isolation